Sentence examples for primers design from inspiring English sources

The mRNA sequences of the five genes were downloaded from EnsemblPlants database (http://plants.ensembl.org/Oryza_sativa/), and were used for primers design using Primer3 software.

To control the false positive signals, the hybridization condition, DNA polymerase, extension time and primers design were optimized.

Additional reaction, improvement of DNA preparation, and/or new primers design are necessary to improve the sensitivity for detection of T. vivax in field samples.

Traditionally, tick-borne pathogen detection has been carried out using PCR-based methods that rely in known sequences for specific primers design.

Nested PCR assay was established based on primers design and applied to seven fecal samples from road killed birds containing nematode and/or trematode eggs.

In spite of including Streptomyces WS/DGAT sequence for TgsX800 primers design (Additional file 1: Fig. S1a) and its positive amplification from a pure culture of Streptomyces coelicolor; no sequences affiliated to this genus were detected in our environmental PCR analyses covering ws/dgat.

The primers designed amplified allS.

Primers designed upstream of the initiation codon amplified the insertion.

Amplification was done by primers designed by PRIMER3 software.

Primers designed with Primer Premier 5.0 are shown in Table 1.

Primers designed with Primer Premier 5.0 are shown in Additional file 1: Table S1.