Sentence examples for primer using an from inspiring English sources

DNA amplification was performed using the HotStar HiFidelity Polymerase kit (Qiagen) and 10 ng of genomic DNA for each strain followed by sequencing of the PCR products with each custom primer using an A3130XL Genetic Analyzer (Applied Biosystems).

Each product was sequenced with the corresponding antisense primer using an ABI Prism® 377 XL automated DNA sequencer (Applied Biosystems, CA) and verified using the Nucleotide-nucleotide BLAST database (BLASTN 2.2.11).

Total RNA was reverse transcribed with oligoDT primer using an Invitrogen SuperScript III kit.

The amplified product was purified and subjected to automatic sequencing using the 5' oligonucleotide as primer using an ABI Prism 310 DNA sequencer (Applied Biosystems).

From each sample, 0.5 μg was used for subsequent cDNA synthesis with the oligo-dT primer, using an iScript Select cDNA kit (BioRad).

Total RNA was extracted from fresh skeletal muscle and liver tissue using TRIzol reagent (Invitrogen) and reverse-transcribed into first-strand cDNA with oligo-dT adaptor primer using an RNA PCR kit (TaKaRa).

Twelve colonies were sequenced with the T7 universal primer using a DYEnamic ET Terminator Cycle Sequencing kit (Amersham).

Random clones were sequenced with the T7 primer using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Inc., Foster, CA).

Equal amounts (1 ug) of RNA were used to synthesize first-strand cDNA with an Oligo dT) primer using a reverse transcription kit (ABgene, Rochester, NY, USA).

Apply your primer using a foundation brush.

Cover the stain with a stain blocking primer, using a throw-away brush or a mini 6 inch (15.2 cm) roller.