Sentence examples for primer to verify from inspiring English sources

A standard nucleotide-nucleotide BLAST (blastn)[26] search was performed on each individual primer to verify the species-specific origin of the sequence in question, followed by the determination of melting temperature using QIAGEN's Oligos Toolkit (http://oligos.quiagen.com/oligos/toolkit.php).

The PCR product was gel purified and sequenced with T7 primer to verify its identity.

All candidate suppressors were then retested to ensure accuracy in visual scoring, tested to see if they were global suppressors of RNAi utilizing combinatorial RNAi with lit-1 and each potential suppressor, and sequenced using an M13 forward primer to verify their identities along with a subset of nonsuppressing candidates (n = 42).

Site-specific primers were designed and used with COKC-end primers to verify the homogeneity of inserted COKC in the specific loci (Table 1).

The DNA was sequenced using both forward and reverse primers to verify the sequences.

The genome is A+T rich and we had to design primers to verify the most of the homopolymer runs.

ARR2PB-CreER(T2) mice [21] were genotyped using the following primers to verify for germline transmission: HPRT-FP: cct tac ctt tca gaa gta ggc and CreER(T2 2: atc aac gtt ttc ttt tcg g.

Randomly selected amplicons were sequenced with the respective primers to verify the identity of the bands.

Ligations were tested by PCR amplification using different combinations of primers to verify that the correct constructs had been produced.

We next designed overlapping primers to verify the sequence obtained from the deep sequencing and assembly processes.

Cloned fragments were sequenced using M13 forward and M13 reverse primers to verify the integrity of the Il-12 p40 sequence.