Sentence examples for primer to generate from inspiring English sources
Besides, two stop codons were placed downstream to the homologous region of each forward primer to generate the specific truncated proteins at the C-terminus.
The activator contains a sulfinic acid salt that reacts with the polyalkenoic acid co-polymer of the primer to generate free radicals, while the catalyst containing benzoyl peroxide increases the self-cure mechanism in dual-cured cement [10, 11].
A specific PDC2 homologous sequence was inserted at the 5′-end of each primer to generate the recombination event at the expected C-terminal region of the PDC2 gene.
Target DNA is first exponentially amplified using error-prone polymerase chain reaction (PCR) and then linearly amplified with a single 3′ primer to generate long, randomly mutated, single-stranded megaprimers.
These primers were used in combination with a common downstream primer to generate RT-PCR products from oocyte RNA (Figure 1C).
At this step, and contrasting to the standard 5' RACE protocol that uses a specific primer per gene, DMTSS employs a random hexamer primer to generate cDNA products for each RNA source.
Another method, single cell bisulfite sequencing (scBS-seq), modifies the Post-Bisulfite Adaptor Tagging (PBAT) to perform bisulfite conversion prior to successive primer extension with oligo1 and oligo2 tagged random primers to generate amplicons [70].
One-step RT-PCR (Qiagen) was conducted using primers to generate nine overlapping PCR products.
The sample material was amplified using Illumina primers to generate sufficient quantities required for hybridization.
cDNA was generated from 1 ug of RNA using Omniscript reverse transcriptase (Qiagen) and oligo-dT primers to generate template for GAPDH quantification and an ORF50-specific reverse primer (5' GGAGAGGGACTACTGTTGG 3') to generate template for ORF50 mRNA quantification.
RT was carried out using SuperScript III (Invitrogen) and gene-specific primers to generate cDNAs according to the manufacturer's protocol.
