Sentence examples for primer to determine from inspiring English sources

100% porcine mtDNA was also run as a control for the AS forward primer to determine whether there was any non-specific primer binding and the degree of specificity and sensitivity of the AS-PCR reaction.

To assess the efficiency of the library, random plaque-forming unites were converted into phagemids and sequenced using the T7 primer to determine the presence of an insert.

Statistical t-tests were performed for each sRNA between the Ct value for the (reverse strand) vs the Ct value of the background (no primer) to determine if there was significantly higher expression than background.

The Renilla luciferase ORF alone was amplified using forward primer 5'-ATGACTTCGAAAGTTTATGATCC-3' and reverse primer 5'-CTCGAAGCGGCCGCTCTAG-3'. SOEing products were then ligated into the pCRII-Topo vector (Invitrogen), digested with EcoRI (Promega) and cloned at the EcoRI site of pcDNA3.1 (Invitrogen) and sequenced with T7 primer to determine the orientation of the cloned insert.

Similar to the GLM in TASSEL, we treated the PCs, location, and year as covariates by forcing their inclusion in the multivariate model, and we implemented forward sequential tests in PRIMER to determine the model with the best fit using the remaining two predictor variables (length and week).

Specific primers to determine the presence of an intestinal fluke, Haplorchis taichui, were investigated using the high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR, and 18 arbitrary primers (Operon Technologies), to generate different polymorphic DNA profiles.

Primers Fg16F (5′-CTCCG-GATATGTTGCGTCAA-3′) and Fg16R (5′-GGTAGGTATCCGACATGGCAA-3′) were used as a pair of specific PCR primers to determine if a Fusarium isolate belongs to a FGSC isolate, and sizes of this PCR amplicons were used to assign the sequence characterized amplified region (SCAR) types to each isolate (Carter et al. 2000; Nicholson et al. 1998; Carter et al. 2002).

Previously published sequencing primers for Pfama1 [19] were used in conjunction with amplification primers to determine full-length sequences (Perkin-Elmer BigDye 3.1).

Serial five-fold dilutions of RNA samples were amplified in quadruplicate by reverse transcription and nested PCR using SIVmac239 gag-specific primers to determine the end point.

Here we use the primers to determine the number of fathers per pod.

Danio rerio ST8Sia sequences identified in silico were amplified by PCR with specific primers to determine expression.