Sentence examples for primer to avoid from inspiring English sources

It was reported that it is necessary to spike an LNA into the reverse primer to avoid aberrant amplification products but this effect was only demonstrated for primers with very high Tm (67 - 68°C ) [ 15].

The 100-fold dilution of the first exponential amplification product before the linear amplification of the second step decreases the concentration of LAD1 and LAD3 primers to avoid non-specific amplification in this linear amplification.

The PCR for integron class I detection was performed using two sets of primers to avoid false negative results due to modifications in the 3'CS region.

Many of these enzymes belong to protein families having similar functions and structures, thus particular care was taken in designing specific primers to avoid cross-amplification of related genes.

We also designed our primers to avoid underlying polymorphic sites.

Some loci were amplified using two sets of primers to avoid repetitive elements.

The samples negative for GP5+/6+ were further tested using CPI/IIG primers to avoid false-negative results.

For most genetic tests, selectivity issues are best avoided by careful design (eg, BLAST primers to avoid nonspecific amplification) or by applying adapted controls and/or limitations.

In the latter cases, a set of alternative reverse primer sequences were used in combination with three different substrate primers to avoid false-negative results.

The amplification cycle was repeated 50 times to ensure complete consumption of the biotinylated primers to avoid background signals during pyrosequencing.

Where these features were not observable, sequences at the 3'-end of the ESTs were used for designing primers to avoid long introns and target less-conserved 3' UTRs.