Sentence examples for primer through the from inspiring English sources
On the contrary, upon binding of the specific target protein onto the primer through the protein-small molecule interaction, the HCR will be inhibited due to the steric and allosteric effect.
This product was used to carry out MassExtend reactions using flanking extend primers, dideonucleotides and Taq polymerase to extend the primer through the polymorphic site.
The control shows ϕ29 Pol efficiently extends a primer through the 3.2 kb duplex and thus produces an ssDNA lagging strand.
The addition of a DNA polymerase along with a mixture of terminator nucleotides allows extension of the hME primer through the polymorphic site and generates allele-specific extension products, each having a unique molecular mass.
The 5′ end of the gene was amplified via PCR using the gene-specific reverse primer ErGPCRF1 and the 5′ primer through the Genome Walker method as described by Clontech Laboratories Inc. (cat. no. 638904; Clontech, CA, USA).
The original primers were modified into sixteen unique forward, and sixteen reverse primers through the addition of 5' dinucleotide tags (Table 1).
Resulting colorspace reads were filtered for quality [ 19] and mapped to the genes used to make the qPCR primers through the corona lite mapping pipeline allowing 2 mismatches and 10 matches for any given read.
In brief, the bisulfite-treated samples (50 ng) were amplified with a biotin-labeled primer through PCR, which enables the conversion of the PCR product to a single-stranded DNA template suitable for pyrosequencing.
A recent paper reported a successful multiplex PCR with more than 1000 primer pairs through the aid of computational design [44].
DNA polymerase is employed with a mixture of nucleotides and terminators to extend from the primer and through the polymorphic site.
It is slightly larger then the region analysed in human and bovine tumours [ 17]. Figure 4B shows the equine CpG island and the position of the amplification primers obtained through the MethPrimer algorithm.
