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We designed a primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB).
In addition, we designed a species-specific primer set to detect M. elsdenii.
Thus, we designed a primer set to detect the fungal nitrite reductase gene (nirK) and validated its sensitivity and specificity.
Positive samples were then tested using a second primer set to confirm the presence of the suspected fusion as described in Table 2.
Our results demonstrated the feasibility of using the serA gene-based PCR primer set to detect and quantify SeRB in environmental samples.
Application of this primer set to genomic DNA from TCBZ-resistant and -susceptible field populations has shown a significant difference in the alleles present.
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LAMP primer sets to detect CCYV were evaluated for their sensitivity and specificity, and a primer set designed from the HSP70h gene with corresponding loop primers were selected.
We designed 5 PCR primer sets to simultaneously detect the β-lactam resistant genes and quantify the pathogen.
However, high sequence variability of the viral agents posts a challenge to the design of robust universal virus-specific primer sets to amplify various virus variants.
Primer sets to generate the probe were: Evi32SPF (5'-AGAGCCCTGGATCTCTAGCC-3') and Evi32SPR (5'-GAGAAAGGGGCGTTTAATCC-3').
For Prdm1, tissues and cells were analyzed with two different PCR primer sets to increase confidence.
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CEO of Professional Science Editing for Scientists @ prosciediting.com