Sentence examples for primer set the from inspiring English sources

When used in conjunction with the newly designed primer set, the selected DNA barcode region was demonstrated to be highly discriminatory, accurate, efficient, and species-specific.

Polymerase chain reaction used consisted of 3 min at 95°C, followed by 30 cycles at 95°C for 15 s and 60°C for 1 min. To assure the amplicon specificity for each primer set, the PCR products were then subjected to a melting curve analysis.

The RNA was used as template for the real-time RT-PCR reaction with the QuantiTect SYBR Green RT-PCR kit (Qiagen) using the p53F and p53R primer set, the mdm2F and mdm2R primer set or the p21F and p21R primer set.

To assure the amplicon specificity of each primer set, the PCR products were then subjected to a melting curve analysis.

Of each primer set the forward primer was labeled with FAM or HEX dye on the 5' end.

For each primer set, the efficiency of the PCR reaction was measured in duplicate on serial dilutions (factor 10) of the cDNA sample.

"In general, a primer sets the stage for an even, smooth topcoat (paint) application.

The NTS1 primer set spans the RFB site, and the NTS2 primer set overlaps the polymerase I transcription initiation region for the 35S rRNA gene.

The first primer set flanks the test region and measures the concentration of deletion-bearing molecules.

However, of those 4 primer sets, only the primer set targeting the tuf gene also amplified DNA from all Shigella strains tested.

Overall, use of the 28S primer set is more suitable than the 18S primer set for the diagnosis of Aspergillus.