Sentence examples similar to primer procedure from inspiring English sources

To overcome the problem of primer-primer annealing observed with the double primer-procedure, we developed an alternative single-primer PCR procedure outlined in Figure 1.

Reverse strand synthesis was carried out using poly dT primers, procedures and reagents from the Superscript II RT kit (Life Technologies).

From the primer validation procedure, primer pairs were grouped into successful or failed, according to the analysis criteria.

All data generated from the high-throughput primer validation procedure can be freely accessed from PrimerBank.

The primer design procedure is crucial for distinguishing one species from others.

The primer walking procedure was repeated until a poly(A) tail or a vector-derived sequence appeared.

We expect that RExPrimer is able to fill the gap and accomplish current needs for automated primer design procedure.

As part of the primer design procedure, PrimerSeq utilizes the biconnected components algorithm [24] as a generalized definition of AS events called alternative splicing modules (ASMs).

Putative full-CDS FLICs selected were sequenced with a "primer walking" procedure in which each sequence read was used to design a primer to extend sequence in the 3' direction.

Using a primer extension procedure used previously for paired-end-sequencing-based decoding (Han et al. 2010), we amplified the barcode sequences together with the flanking genomic DNA sequences from the genomic DNA of pooled mutants.

Although these studies demonstrated that the number of unique tags does not necessarily need to match the number of samples, they considerably increase the complexity of sample handling by employing experimentally complicated sample or primer mixing procedures.