Sentence examples for primer obtained from from inspiring English sources

The cDNA was PCR amplified using adapter 2 as the forward primer and a gene specific reverse primer obtained from the N gene (5'-GTTTAATCTTAAGAGCCCTCCTGTC-3'), which would prime at APMV-5 nt 200 224, within the N gene.

Primers obtained from the Primer Bank [34], [35], [36] are given with the PrimerBank ID Accession number.

Exons were re-sequenced for polymorphisms with primers obtained from DOGSET [35].

cDNA was synthesized from total RNA by using miRNA-specific stem-loop primers obtained from commercial service (Takara, Dalian).

For the PCR reaction, specific primers obtained from IARC TP53 Mutation Database website (http://www-p53.iarc.fr) were used for each exon (exons 2 to 10).

Sequencing primers were typically universal primers obtained from ABI or internal primers, as mentioned above.

The nuclear ITS1 locus was amplified with primers obtained from Armbruster et al. [ 38].

Forward and reverse oligonucleotide primers, obtained from Invitrogen (Paisley, UK), were 5-ccagggctggacgccaccaccacc-3′ and 5′-ctggggtcactaaagcatgcactggtcagg-3′.

CYP7A1 expression was normalized to the expression levels of 2-microglobulin using primers obtained from the same company.

The ΔCt was calculated by subtracting the average Ct value of each tissue type from the average Ct values of 18S rRNA (ribosomal primers obtained from Applied Biosystems, Foster City, CA).

PCR and DNA sequencing primers were obtained from Bioneer; primer sequences and conditions are available from the authors upon request.