Sentence examples for primer mixed from inspiring English sources

Diluted cDNA samples (1/10) were used for Real time PCR analysis with 200 mM of each gene specific primer mixed with SYBR Green PCR master mix in a final volume of 15 μl following manufacturer's instructions.

Using this method 16 additional mice received hydrogel primer mixed with Cy5 labeled fluorescent oligonucleotide (10 to 100 microg).

Strengthening of these areas consisted of first preparing a batch of primer mixed with sand and applying grout mixture to the three areas of strengthening.

Briefly, 40 μg total RNA from naïve animals and 50 μg from pristane-treated animals were pre-annealed with a poly dT primer, mixed with reverse transcriptase, deoxynucleotides, RNase inhibitors and dUTP-Cy3 or dUTP-Cy5.

Multiplex PCR was conducted in a 10 µl reaction mixture containing 4 ng plant genomic DNA, 10 pmol of each primer mixed as designed in the MPMP table, 1× KAPATaq EXtra buffer (without Mg2+), 1.75 mM MgCl2, 0.3 mM of each dNTP, and 0.25 U of DNA polymerase (KAPATaq Extra, KAPABIOSYSTEMS, Boston, MA, USA).

Diluted cDNA samples were used for Real time PCR analysis with 200 nM of each primer mixed with SYBR Green PCR master as per manufacturer's instructions.

For the NSR-primed cDNA synthesis, 2 µL of 100 µmol/L first-strand NSR primer mix were mixed with 1 µL of total RNA, 1 µL diluted Spike-In Mix and 6 µL of nuclear-free water in a PCR tube.

To evaluate the efficiency of reverse transcription (RT) reactions using different number of primer, we first compared the use of a single miRNA specific primer and an 11 primer-mixed set using 100 ng total RNA isolated from A549 and H1299 lung cancer cells.

Fluorescently labeled primers were mixed with unlabeled primers at a 12 20 ratio.

The five sets of species-specific primer were mixed and applied to simultaneously detect bonito species.

Briefly, enriched ion sphere particles (ISPs) were annealed with the sequencing primer and mixed with the sequencing polymerase from the Ion PGM_200 Sequencing Kit (Thermo Fisher Scientific).