Sentence examples for primer link from inspiring English sources

The search results provide a primer link that leads to the design of the primer for the corresponding repeat type.

The linker was generated from the scFv A7 isolated by phage display [ 42] by a PCR with the primer LINK BACK: 5'-GGC ACC ACG GTC ACC GTC TCC TCA and LINK FOR 5'-TGG AGA CTG AGT GAG CTC GAT GTC.

Briefly, a pool of mRNA samples was converted into cDNA using biotinylated oligo dt) primer linked to magnetic beads.

was used with an oligo dT) primer linked to the T7 RNA polymerase-binding site sequence to synthesize cDNAs.

We converted RNA to double-stranded cDNA using Superscript Reverse Transcriptase (Invitrogen) and an oligo-dT primer linked to a T7 RNA polymerase binding site sequence.

First, 300 ng-3 μg of total RNA with the appropriate concentration of RNA spike-in controls was converted into double stranded cDNA using an oligo (dT) primer linked to the T7 promoter sequence and MMLV-RT.

After PCR amplification with adapter primers linked to the MLPA-probes, the methylated regions amplify and are detected while the digested unmethylated regions fail to amplify[22].

The remainder of the genome was determined from RNA of poult 2993D by RT-PCR with primers linking the individual contigs and singletons.

The PCR was carried out using primers linked with fluorescent dyes (6-carboxy-4',5'-dichloro-2',7'-dimethoxy-fluorescein, fluorescein, and carboxy-tetramethylrhodamine).

In the standard protocol [ 16], an initial cycle of reverse transcription (RT) converts RNA into cDNA using random primers linked with the T7 promoter.

Error bars indicate RQ max and RQ min. Templates of cDNA for axolotl patched- 2 319 bp) and pax7 (303 bp) were amplified by RT-PCR using respective sense primers linked to SP6 promoter sequence and anti-sense primers linked to T7 promoter sequence.