Sentence examples for primer into the from inspiring English sources

The result is as good a primer into the current state of the psychiatric world as you will find.

We anchored when possible the right-hand primer into the 3′-UTR and the left-hand primer into the coding region.

To incorporate this randomized oligonucleotide primer into the H1 promoter, we used it as an antisense PCR primer along with the sense primer H1-5.1, 5'-AGTGGCGCCCTGCAATATTTG.

In the presence of PCNA, PolB1 activity is stimulated by 50% (as measured by full-length DNA synthesis), further extending the primer into the region occupied by the downstream Okazaki fragment (lane 4).

Rather, it is both a primer into the basic physical principles underlying brain function and a window into a number of problems and challenges that the authors found to be of current interest.

Galc endogenous mRNA was amplified using forward and reverse primers into the mouse gene sequence (NM_008079), whereas for the transgenic one, we designed a reverse primer into the vector construct WPRE sequence (forward: 5′ GAC TGT GCA GTG CGA TGT TT 3′; reverse Galc: 5′ GCC CTG AAC CAA AAT CAA AA 3′, reverse WPRE: 5′ AAG CCA TAC GGG AAG CAA TA 3′; annealing temperature, 56°C).

Hunter was carefully pipetting various fragments of other DNA, called primers, into the top of the machine.

Expression vector plasmids for the PTD fusion proteins were constructed by inserting the cDNA fragments encoding PTD, which was prepared by annealing the corresponding oligonucleotides, ODD and EGFP, which were prepared by PCR amplification with the corresponding primers, into the plasmid pGEX-6P-3.

Y. pestis strains overexpressing one of each of the ten GGDEF/EAL/HD-GYP genes (Table 1) were made by first cloning a wild-type copy of each gene, PCR-amplified from KIM6+ using specific primers, into the high-copy number plasmids pUC18 or pCR2.1-TOPO (Invitrogen).

The resulting amplified crossover PCR fragment was cloned using flanking HindIII sites incorporated in the primers, into the HindIII site of pBB400 (Table  1) [ 26].

These results demonstrate an efficient partitioning of the participation of the LS and NLS primers into the first and second stages of PCR, respectively.