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Pour latex primer into a paint tray.
Pour primer into a paint tray.
Pour the primer into a bucket or paint tray.
Pour your primer into a painting pan or bucket, and remove any packaging from your paintbrushes.
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We incorporated the species-specific primers into a conventional multiplex PCR assay and assessed its performance with a collection of 97 Enterobacteriaceae strains.
MA plots, Pearson correlations, and log fold change plots were made using R. Transcripts identified by differential expression were cloned using forward and reverse primers into a double-stranded RNA expression vector as previously described (Rink et al., 2009).
A 5' anchor, a T7 polymerase binding sequence, and a random four nucleotide sequence (CGAAA-TTAATACGACTCACTATAGGGAGA-NNNN-7mer) were added to the primer sequence in order to integrate the genome-directed primers into an Eberwine-based amplification system.
Briefly, total RNA (25 μg) was reverse transcribed, using an oligo-dT primer, into cDNA incorporating an amino-allyl modified d-UTP, which was then labelled with either Cy5 or Cy3 fluorescent dyes and hybridised to the microarray for 16 h at 42-C.
The high specificity of this approach is achieved at two levels: the use of a reverse transcription primer folded into a stem loop structure with optimal energetics and the use of specific PCR primers to the loop structure.
βCCNB1, βPTCH2, βAKIRIN1, βTHOC2, βWDR13, and βCCNB1/A/B and C expression plasmids were made by insertion of genomic PCR fragments, isolated using gene-specific primer sets, into a cloning vector prepared by long range PCR amplification (Barnes, 1994) of βΔTERM with primers BETA43/BETA10.3 using PrimeSTAR HS DNA polymerase (Takara).
Rather, it is both a primer into the basic physical principles underlying brain function and a window into a number of problems and challenges that the authors found to be of current interest.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com