Sentence examples for primer inserted from inspiring English sources

The primer inserted an additional GSGS spacer between the two LIRs.

To allow expression of the protein, an artificial start codon (ATG, shown in bold in the primer sequence) was added on the forward primer, inserted in a Kozac sequence.

These primers inserted, respectively, Sac I and Bam HI sites upstream and downstream of the cDNA fragment.

These primers inserted, respectively, Sal I and Bam HI sites upstream and downstream of the promoter sequence.

The CAH1 forward oligonucleotide inserts an Xho I restriction site, the CAH1 reverse primers inserts an Xba I restriction site (both restriction sites are in bold).

Medaka myc17 (ENSEMBL database identifier: ENSORLG00000007021) and myc20 sequences were PCR amplified from pooled medaka organ cDNA using ReproFast polymerase (Genaxxon) with primers inserting a HindIII restriction site downstream and a BamHI site upstream of the gene (for primer sequences, see supplementary material Table S3).

pAPs-VN and pAPs-VC: the encoding sequences of SARS-CoV accessory proteins were amplified by individual primer and inserted into the SalI-digested vectors pGAL10CYC1-VN and pGAL10CYC1-VC to produce BiFC vectors pAPs-VN and pAPs-VC (Figs. S3 and S4 in Supporting information).

Two NHase genes, NHBA and NHBAX, from Nocardia YS-2002 were successfully cloned, based on bioinformatics design of PCR primers, and inserted into plasmid pUC18 and pET32a, respectively.

The Maf1 coding region from a human cDNA encoding full-length human Maf1 (GenBank accession number NM_032272) or Maf11-81 and Maf11-142 fragments were amplified by PCR using specific primers and inserted into various vectors.

For expression of the GST-SLPI W1 and GST-SLPI W2 fusions, PCR fragments obtained using pLex-SLPI as a template and appropriate primers were inserted into the EcoRI-XhoI sites of the pGEX4T-1 plasmid.

Mouse cDNA for IRF3 was amplified from MEF cells using gene-specific PCR primers and inserted into the XhoI/NotI site of the pME vector with an N-terminal FLAG tag.