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Slashes indicate cutting sites for Kpn I and Bam HI restriction enzymes, and lower cases in 5CquiOBP1-KpnI primer indicate bases replaced to overcome codon bias of E. coli and thus enhance protein expression.
Therefore, the data obtained here, whether considered as a library based on the multiple PCR primer approach, or as a collection of libraries each based on an individual PCR primer, indicate a surprising level of protistan richness in the Arctic community.
The 229 bp product of wild-type specific primer and reverse outer primer indicate the presence of wild-type allele in the sample.
Similar(57)
Even if a token offering were to avoid 'security' status under the Howey Test, the CFTC's primer indicates the token could still be subject to CFTC jurisdiction.
Apparently the same Operon oligos behave differently in combination with a different primer indicating that the choice of the primer is causing these differences.
Polymerases were aligned on the 3' end of the primer, indicated by the solid line.
The lowercase letter in the primer indicates a mismatch nucleotide being introduced to increase the primer specificity [16].
Primers' names are given according to the Table 1, first and last nucleotide position of each primer is indicated, lines between primers indicate the PCR product of the length counted.
Underlined letters in the primers indicate the nucleotides that were changed.
*Nucleotides in bold letters in primer sequences indicate primer modifications included in this study.
Results: All cases were positive for the universal bacterial primers, indicating that samples contained bacterial DNA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com