Sentence examples for primer in order from inspiring English sources

750 1000 ng of total RNA was then used to make the 1st strand cDNA using Superscript III (Invitrogen) with either oligo-dT primer for cellular transcripts or with DENV-qR primer in order to amplify the DENV genomic RNA.

The GC-clamp, which is a sequence that is rich in guanine and cytosine, is added to the 5′ end of the forward primer in order to prevent DNA from being completely denatured into single strands and to improve band resolution in denaturing gels.

After killing the mildew, it's a good idea to add a coat of shellac or stain-killing primer in order to keep the mildew from growing again in the same spots.

A Kozak sequence was added before the first ATG of each forward primer in order to increase protein expression.

We suggest to introduce a forth primer in order to capture all the possible mutations.

The T7 promoter was added to the forward primer in order to generate a sense strand of the RNA molecule.

The first PCR step is conducted without primers in order to reassemble the DNase I-digested fragments into longer pieces.

Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome.

In the present work, we propose a rational method of designing primers in order to amplify the variable region of heavy chain (VH) and variable region of light chain (VL) domains for framework 1 (FR1) of immunoglobulins.

Partial gene sequences of the phenylpropanoid and flavonoid biosynthesis pathways were isolated from the native Chilean strawberry fruits, and used to design gene-specific primers in order to perform transcriptional analyses by qRT-PCR.

Generally, an efficient RT-PCR relies on finding the most conserved sequences across all virus genotypes to use as primers in order to efficiently amplify the maximum number of these diverse genetic variants.