Sentence examples for primer encoding from inspiring English sources

In the second step, a PCR product was generated using activated Ack1(L487F) mutant (Mahajan, 2005 #12) as the template and the reverse primer encoding a Myc-tag.

The 137-nt RNA template was prepared by in vitro transcription using a double stranded (ds) PCR product amplified by a 37-mer forward primer encoding T7 promoter sequence and 19-nt of the viral sequence and a 16-mer reverse primer encoding 3' sequence of the PA gene.

CaBP7 tagged with c-myc at the carboxy-terminal end (mCherry-CaBP7-myc) was made by PCR sub-cloning using wild-type CaBP7 template and previously described sense primer [15] in combination with a reverse primer encoding the ten amino acid sequence (EQKLISEEDL) of c-myc [23]: 5'-AACCAGGTGCTGCGCAGTGGCATGAAGGAACAAAAACTTATTTCTGAAGAAGATCTGTAGCCGCGGGCCCGGATAT-3'.

The FPs were amplified with a 5' primer encoding an AgeI site and a 3' primer encoding either a BspEI (C1) or Not1 (N1) site.

Cerulean was PCR amplified from plasmid DNA using a forward primer encoding a 5′ HindIII restriction site followed by a Kozak consensus sequence for mammalian expression (Kozak, 1987) and a reverse primer encoding a 3′ BamHI site.

The 3′-megaprimer was constructed using the T7term primer and a sense primer encoding the desired mutation.

The 5' and 3' primers encoded EcoRI sites, and 5' primer encoded an ATG initiation codon embedded into an optimal Kozak's sequence.

The forward primer encodes the flag tag.

Because the forward primer encodes CDR3, the resulting PCR product encodes a V-domain mixture with subtly variable CDR3s and hence fine specificities.

The central part of the SM132 primer encoded for the (SGGG 3S linker (in bold) and contained an amber codon (in italic) and SpeI restriction site (lowercase letters).

This primer encoded an AUG translation initiation codon followed by the codons for the eight amino acids in the FLAG epitope (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) and six amino acids from nucleophosmin/B23.