Sentence examples for primer during the from inspiring English sources

Before CHINICT and GMIC took place, Benjamin Joffe from Asia-based digital strategy consultancy +8* | Plus Eight Star delivered a primer during the Startup2Startup Beijing event on how innovation in China works, and how local companies adjust proven business models from abroad to meet the peculiarities of the Chinese market.

We have recently listed the critical technical points to amplify these low-abundance and GC rich mRNAs, and highlighted the need for a higher temperature and a gene-specific primer during the RT step [21] (see Materials and Methods).

We show that accurate quantification of a specific strand of viral RNA, in the presence of relatively higher levels of cDNAs generated from the complementary strand, requires incorporation of a unique tag sequence into cDNA generated during the RT step, and the use of a tag-specific primer during the qPCR step.

The model of the thermal cycler was not changed for a particular primer during the course of the study.

CTAGATG duplex melting temperature (Tm) is −1.64 °C, which makes it practically impossible to use it as a sole priming region for the forward primer during the PCR.

In both human and mouse samples, we identified methylated cytosines in the promoter region of the heavy strand (PH) and within conserved sequence blocks (CSBI-III), which are highly conserved sequences located at the 5′-end of the D-loop and considered to be implicated in the processing of the RNA primer during the replication of the H-strand.

By using one specific primer and one reverse primer during PCR, the precise sequence of the end of the miRNA opposite to the specific primer could be correctly amplified and validated.

In vitro, Y477F mutant loses its sensitivity to the newly discovered inhibitors but is unable to extend primers during the elongation phase.

Since we used oligo dT primers during the reverse-transcription, this aggravates low expression levels.

The relative expression levels of eight chloroplast-encoded genes considered to be of great importance during the photoacclimation process were investigated by qRT-PCR using random primers during the cDNA synthesis.

The replication factor consists of the DNA synthesizing DNA polymerase α and the RNA synthesizing DNA-dependent RNA primase; both enzymes are required to synthesize the RNA/DNA primers during the initiation and elongation process of DNA replication.