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The primer data are given in Table 3.
The gaps (space positions) in the primer data matrix X(0 N×M×L may interfere with the results of statistical analysis badly.
After multiple sequence alignment the protein family is represented by a three dimensional primer data matrix X(0 N×M×L.
After unnecessary gaps are deleted, we get the reduced data matrix XN×M×L and amino acid position frequency matrix FM×L, in which the sequence length L is smaller than in the primer data matrix.
Primer data are indicated in Additional file 1: Table S2.
Primer data are presented in Additional file 4: Table S2.
Similar(42)
Although bioinformatics (http://rice.plantbiology.msu.edu) shows that there is another transcript, we haven't detected it by RT-PCR method using specific primers (data not shown).
This was further confirmed by PCR using tet(O) specific primers (data not shown).
The PBS contained no detectable bacterial contamination as determined by PCR using bacterial 16S specific primers (data not shown).
However, we were unable to detect stable interactions between the TEN domain and telomeric ssDNA primers (data not shown).
Partial sequencing of Mus spretus G6pdx cDNA overlapping exons 6 to 13 confirmed the absence of SNPs in G6pdx#1 primers (data not shown).
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