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In gynaecological samples, PCR results were similar independent of the primer combination used.
The optimal annealing temperature for detection of T. harzianum strains was from 62° to 63°C with this primer combination.
PIC was averaged over the fragments for each primer combination.
The percent of reproducibility was calculated for each primer combination.
The primer combination E36xM34 produced the highest number (194) of fragments, while the primer combination E36xM33 produced the least number (32) of fragments (data not shown).
Primer combination for each PCR was shown on the gel images.
The primer combination E36xM34 displayed the highest number (510) of fragments in population AA in total of eight individuals, while the primer combination E39xM41 displayed the least number (81) of fragments in population AI in total of six individuals.
Primer combination of the Gn1a-17SNP marker, its predicted band size, and deduced gel image depending on genotypes were presented.
Primer combination for each marker was shown on the gel images and its sequences were listed in Table 2.
Number of loci counted for each primer combination varied from 10 to 70 (Table 2).
PCR cycle details are provided per primer combination in Table S2.
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