Sentence examples for primer causes from inspiring English sources

We speculate that the 3DA substitution at position n – 2 in the primer causes a reorientation of the K706 side chain, which prevents a steric clash between the ε-amino group of K706 and the C3 hydrogen of 3DA.

The Co acac 2 primer caused the increased adhesive property with the enhancement of molecular interaction, chemical affinity, and mutual diffusion.

In this study, preparation of hypomineralised enamel with Transbond Plus self-etching primer caused significantly higher microleakage at the adhesive-bracket interface than when the conventional acid-etching or NaF treatment were used.

Compared with the all-DNA primer (Table 2), the 3'-gT primer caused 2 4 fold decrease in kcat (Table 3).

One contained a single nucleotide deletion in the primer region, indicating that impurity of the synthesized primer caused the production of an undesired product.

Specifically, the use of a "universal" set of ASPCR primers caused an overestimation of the K103N (ntAAC) mutation at position 103 of reverse transcriptase when primer binding site polymorphisms resided close to the 3′ end of the allele-specific primer.

The primers cause only certain short segments of DNA to replicate.

The use of selected primers caused the desired reduction in rRNA reads non-ribosomal reads improving from 11% with random hexamers to 45% with selected hexamers.

Most of these discrepancies (2.1% -heterozygous only in SNPlex; class I) reflected a preferential annealing of PCR primers caused by polymorphism (SNP or In/del) in the PCR priming site, which was identified by sequencing.

4 not determined Since mismatches in primers cause a shift in amplification profiles we calculated the differences between quantification cycles (Cq) generated by reactions containing primers with mismatches to the average Cq obtained with perfect match primers (ΔCq method; see Additional file 1).

Moreover, some spacers (particularly 37, 38, and 40) are missing in certain Beijing/W isolates because of loss of the target for DRa and DRb primers caused by deletions or presence of IS 6110 insertions in the DR region (32, 33 ).