Sentence examples for primer back from inspiring English sources
A look inside Dan & Whit's General Store in Norwich provides a quick primer: Back in the hardware department the other day, a sinewy woodsman with Phish-y facial hair wearing a chambray workshirt, jeans and Timberland boots compared prices of motor oils while his wife -- braless in a tank top with graying bangs and a three-foot-long braid -- surveyed the organic produce.
So perhaps the biggest reason why the practice is not more common is that even though there have been numerous articles published on the topic Nate Silver wrote a primer back in 2011 it is still something of a trade secret among the most dedicated fliers.
Additional tools were developed to parse EMBOSS primersearch output for conducting electronic PCR and PATMAN [ 37] for mapping primers back to sequences.
This was performed with the primers BACK-1, BACK-2, and PMCF (Table 1) containing restriction enzyme sites Pst I and Not I, using the same protocol as the first PCR but reduced to 17 cycles.
A set of four specific primers was successfully designed to recognize six distinct genomic sequences of UL6 protein from DPV, including one forward inner primer, one back inner primer and two outer primers.
The linker was generated from the scFv A7 isolated by phage display [ 42] by a PCR with the primer LINK BACK: 5'-GGC ACC ACG GTC ACC GTC TCC TCA and LINK FOR 5'-TGG AGA CTG AGT GAG CTC GAT GTC.
In the first reaction equimolar amounts of the DNA of the VH and VL domain and the synthetic linker were incubated with 25 pmol of the primer VH1 Back and MJK1FONX in a PCR of 50 μl containing 1.0 mM dNTPs, 2.5 mM MgCl2 (Qiagen), 5 μl 10 × PCR buffer (Qiagen) and 1 μl (1 U) Taq Polymerase (Qiagen).
The gene for the VHH domain of about 400 bp (Figure 2B) was amplified with nested primers PMCF, BACK-1, and BACK-2 using as template the 600 bp PCR fragment.
Here, a double-stranded oligonucleotide consisting of two primer sequences in back-to-back orientation was designed as an adapter for LM-PCR.
The entire mtgenome of both NAF and corresponding blood samples were amplified using a single back-to-back primer set or three overlapping primers (see primer sequences in Table 1).
Previously reported procedures for whole-plasmid, mutagenic PCR using the entire plasmid as template and back-to-back primer set resulted in linear DNA products, necessitating a separate ligation step to generate circular plasmids before transforming the host cell.
