Sentence examples for primer at a from inspiring English sources

This book offers a succinct and accessible primer at a time when breathtaking technological advances promise a wealth of new observational data on the first stars and galaxies.

It is especially important to remember to apply primer at a festival.

The PCR mixture contained 12.5µl of HotStarTaq Mix (Qiagen), 0.5µl of each primer at a 20µM concentration, and 2µl bisulfite modified DNA.

Since we wanted the amplification to be attributed to only one primer at a time, we generated single-primer templates.

Following successful recovery, the barcodes were quantified using a Genome Analyzer IIx (Illumina) and the GexSeqN sequencing primer at a final concentration of 500 nM.

Reaction volumes of 25 μl contained 0.25 μl enzyme mix, one forward and one reverse primer at a final concentration of 400 nM and 5 μl (1 pg to 1 μg) RNA sample.

The 10μl reaction mix contained 0.5 units of AmpliTaq DNA polymerase (Perkin Elmer) with manufacturer's buffer in the presence of 0.4mM dNTPs and both unlabeled primers at a concentration of 0.22μM, and one end-labeled primer at an approximate concentration of 0.06μM.

The primers (at a final concentration of 10 mM) were designed with the software Primer 3 and listed in Table 1.

PCR reaction mixtures contained primers at a concentration 10 µM and PCR mix (SYBR GreenER qPCR Supermix for iCycler, Invitrogen) in a volume of 25 µl.

Each PCR reaction contained sense and antisense primers at a concentration of 200 nM and the relevant UPL probe (Universal Probe Library, Roche) at a concentration of 100 nM in a final volume of 12 µL of FastStart Universal Probe Master ROXX) Reaction Mix (Roche).

20 µl qPCR reactions were set up using 1 µl of cDNA, 10 µl of 2× SYBR Green qPCR master mix (Applied Biosystems), 2 µl of 5M betaine (Sigma) and primers at a final concentration of 0.2 µM.