Sentence examples for primer as previously from inspiring English sources

DNA fingerprints were analyzed by arbitrary primed PCR using the PMAV17B primer, as previously described [10].

PCR reactions were performed in a final volume of 20 µl, containing master SYBRGreen (Roche Diagnostics, Meylan, France) and 300 nM each HMGB1 primer, as previously described [49].

MiRNAs were quantitated by SYBR-GREEN based quantitative Real Time-Polymerase Chain Reaction (q RT-PCR) on cDNA generated by reverse transcription using a specific stem-loop primer as previously reported.

VH gene family-specific PCR amplification was performed using six family- specific VH primers and one JH primer as previously described (Li et al, 1999).

Testing with One-Step RT-PCR (QIAGEN, Valencia, CA, USA) is performed with region C specific primer, as previously described (8 ).

Total RNA was isolated and reversed-transcribed with Takara Prime Script Reverse Transcriptase and an oligo dT primer, as previously described [ 30].

The entire mitochondrial genome was amplified using 36 overlapping primer pairs as previously described (33).

The ssDsbA was synthetized by overlapping-PCR of four primers as previously reported (Steiner et al. 2006).

PCR-DGGE (denaturing gradient gel electrophoresis) analysis was used to evaluate the diversity of methanogens in each co-culture, using the 519f/915rGC primers as previously described (Cheng et al. 2009).

qPCR was performed using primers as previously described [33].

Genotyping was performed with polymerase chain reaction (PCR) using primers as previously described by [48].