Sentence examples for primer around the from inspiring English sources

The antisense strand-specific RT primer around the DMR failed to produce any product while the sense strand-specific primer was used as a positive control to show that the gene itself was expressed in this region.

Spread the primer around the small chip with a tiny brush.

PCR was carried using previously published primers around the −2 MC2R promoter polymorphism (11).

Primers around the MMP1 polymorphism were 5'-TCTTTGTCTGTGCTGGAGTA-3' and 5'-CAATTTCCTCATCTAAGTGGCATA-3'.

We therefore designed long PCR primers around the gene, avoiding high A+T regions, to amplify two overlapping fragments spanning ∼5.9 kb total (primer sequences below).

The last 18 gaps were bridged by developing primers around the gaps, and traditional Sanger sequencing to yield the final complete cp genome.

We designed a pair of PCR primers around the candidate repeat region in the SCA31 sample the right boundary of which could be determined.

Genomic fragments corresponding to the der(5) and der(8) chromosomes were amplified by primers around the predicted breakpoints on both chromosomes.

Partial cDNA sequences were obtained by using degenerated primers around the highly conserved copper site [16], which were completed by RACE techniques.

A total of 72 geneticin resistant callus lines (UI) were isolated, each of which was subjected to PCR using primers around the Bar gene (Fig.  3a-b).

As SINEs tend to be inserted close to genes in euchromatic regions [ 74, 75], amplifications with primers around the SINEs were used to detect possible co-transcripts.