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The results of the DOE study indicated that ambient temperature, humidity, the primer application process, and the application of a pre-treating heat process positively impact bond quality.
Figure 3 illustrates the primer application.
For cementation to zirconia without separate primer application before luting, bonding efficacy depends entirely on the luting agent and its mechanical stability [24].
Besides, Nb2O5 presents the ability to diffuse into the hybrid layer when inserted into an adhesive resin applied following the etching and primer application in a 3-step etch-and-rinse adhesive system [16].
Open image in new window Fig. 3 Schematic of anchor fabrication and installation procedure:a anchor for system M,b anchors for systems S and F,c surface preparation, primer application, and drilling;d FRP sheet application and anchor, ande finished FRP anchor and sheet installation.
Each figure shows baseline photos (before use of the primer) and 12-week photos (without primer application and following primer application).
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In four pairwise primer applications gene fragments of up to 1500 bp in size could be isolated, which comprise nearly complete β-tubulin genes from twelve species representative of the Fungi.
In the present study, only one species of platyhelminth (Leptoplana tremellaris) was attempted for amplification and sequencing, however, the standard COI-5P was successfully obtained, which may reveal a promising trend for the primers' application to other flatworms.
The primer design application within LIMSTILL is Primer3-based and parameters are set to design primers with an optimal melting temperature of 58°C.
This work presents a web-based AS primer design application called WASP.
We presented WASP, a web-based allele specific primer design application.
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