Sentence examples for primer and was from inspiring English sources

Sanger sequencing was done using the B2M_R primer and was compared to normal control sample as a negative control.

One told me that he'd read Dickey's book over the winter, as a primer, and was amazed to discover that Dickey sounded even more articulate when sweating, after workouts, than on the page.

Each reaction contained 10 to 100 ng of DNA, 10 mM Tris HCL (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 2.5 units of Taq DNA Polymerase (Perkin-Elmer, Norwalk, CT), 150 μM of each dNTP, and 0.3 μM of each primer, and was amplified with a cycling profile of 45 s at 94 °C, 45 s at 48 °C, 1 min at 72 °C, for 35 cycles.

Single-stranded cDNA was generated using a T7 oligo dT) primer and was followed by second-strand cDNA synthesis.

An M13 sequence tag was added to the 5′ end of each degenerate primer and was used for sequencing.

Primer extensions revealed that pol ν could not fully extend the −6 primer and was severely blocked one nucleotide prior to the adducted site.

Even the interior wall primer and paints are free of volatile organic compounds.

The mismatched 3′ terminus between forward primer and template are shown in red.

The biotinylated primer was 5' -andCCAAACtheAAAATCACTCATTA-3' and the pyrosequencing primer was 5' -TAGGAATTTTTTTTGTTTTA-3'.

Primers and sequences are listed in Supplementary Table S1.

All primers and probes are listed in Supplementary Table 3.