Sentence examples for primer and using from inspiring English sources

PCR reactions were performed with 0.3 μM of each primer, and using the following cycling program: 94° for 5 min (denaturation step), 5 cycles at 94° for 1 min, 58° for 45 s, and 72° for 45 s, followed by 25 cycles of 94° for 45 s, 58° for 30 s, and 72° for 45 s.

Unless otherwise indicated, PCR amplifications were performed in 40 μl reactions at standard concentrations (1.5 mM MgCl2, 0.2 mM dNTP, 1× Biolase PCR buffer, 0.5 U Taq (Bioline USA, Inc., Randolph, MA), 0.5 uM each primer) and using standard cycling parameters (94°C - 3 min, (94°C - 30 s, 55°C - 30 s, 72°C - 60 s) × 30 cycles, 72°C - 3 min) then cloned into Topo PCR 2.1 (Invitrogen).

In this study, we designed a novel 5′-amino-modified primer and used it for priming cDNA synthesis.

Forward primers were tagged with a fluorescent dye (universal M13 forward primer) and used in a multiplex PCR kit (Qiagen Inc).

Both 3'- and 5'-RACE reactions were run on diluted adapter-ligated cDNA template employing 0.2 µM of gene specific primers DN1F (5'-ATA CGA CAC CCG TGA CCT GGC TGA A-3') or DN4R (5'-TGT CCT GGG CGA GGC CAA GAT AGA T-3') in combination with 0.2 µM of the AP1-primer and using the Advantage 2 polymerase Mix and buffer provided in the Marathon kit.

Be sure to clean brushes/rollers extremely well after using primer and before using those tools for paint.

To achieve this aim, we designed long primers and used nested inverse PCR to amplify the DNA flanking sequences.

The variant-bearing region was amplified by using specific primers and used in Sanger sequencing (Additional file 14).

Try eye primer and use on your tear duct!

Individual positive clones were isolated by PCR using M13 primers and sequenced using ABI standard protocols.

If necessary, internal primers were designed and used in primer walking.