Sentence examples for primer adjusted from inspiring English sources

Briefly, 2.5 μg dscDNA was mixed with 3 μl (0.5 μg/μl) random primer, adjusted to 23 μl, boiled at 95°C for 5 minutes, and put on ice.

Less than 5 μg of total RNAs were mixed with an appropriate amount of the standard RNAs and 0.5 μg of dT18 primer, adjusted to 10 μl with ddH2O, heat-denatured at 70°C for 5 min, and chilled on ice.

Subsequent PCR amplification and detection of template was carried out using Applied Biosystems transcript-specific assays including: COL1A2 (ID- Hs01028971_m1), COL3A1 (ID-Hs00943793), ACTA2 (ID- HS00426835_g1) and CTGF (ID-Hs00170014_m1) using 15 ng of cDNA and 20x final concentration of Gene Expression Mix, which contains both forward and reverse primers adjusted to final volume of 15.0 μl.

In multiplex assays with three forward primers, each primer was adjusted to 100 mM.

The final concentrations of the T-DNA-specific primers were adjusted to 0.4 μM and those of the AD primers were 3-4 μM (depending on the degree of degeneracy) in the primary reaction and 2 μM in the secondary and tertiary reactions.

The concentration of amplification primers was adjusted to obtain balanced amounts of PCR products in 8 mM MgCl2.

Rapid diagnosis of Escherichia coli (E. coli), Salmonella and Vibrio cholera pathogens can be done with simultaneously in a single multiplex PCR assay by using specific primers with adjusted PCR conditions.

Once primers were adjusted for consistent amplification in both the South Bend and the Johannesburg colonies, they would usually work well for genotyping the field collections.

Two primers were adjusted based on sequences form other tetranychid taxa available from GenBank (Table 2).

The location of the reverse primers was adjusted to make the lengths of RT-PCR products 250-350 bp.

Methods and Results: The new primers were adjusted to separate PCR amplicons (70 to 170 bp) on precast Spreadex gels using horizontal gel electrophoresis.