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Accordingly, a PCR method using a nonspecific primer MINf combined with a primer modified from our 16SFI primer, i.e. the primer MINr, was developed and displayed better detection specificity [Int. J. Food Microbiol. 80 (2003) 67].
Promising resultants were also achieved for the DNA amplification directly performed on magnetic beads by using a novel magnetic primer, i.e., the up PCR primer bound to magnetic beads.
Next, we utilized the ovi R 500con primer (i.e., the reverse primer in the DIAL region) and the reverse complement of the ovi F 230con primer (i.e., ovi R 230con, GATTGTGCCCCCGACGAT, which is in the IVGG region) as the gene-specific outer and inner primers, respectively, for 5'RACE for each tissue within each sex.
All bases beyond the reverse sequence primer (i.e., adaptors and sequencing primers) were removed in this step.
There are four mismatched bases in the forward primer, three of which are in the 3´end of the primer (i.e. 3 mismatches in the last 9 bases of the primer).
I swear by Laura Mercier foundation and primer; I wear eyeliner and mascara, and eye shadow in the evenings.
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From the recent NYT's review, in their Father's Day primer: Is Dad technical?
Furthermore, PCR products for possible excision of McCIIMD0819 in strain IMD0819 were obtained using divergent (primers g and h) and convergent primer (primers i and j) pairs placed inside and outside of McCIIMD0819, respectively.
mPing was amplified with Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific) using the control mPing primers (mPing F and mPing R) or mutation containing primers (i.e., mPing F and mPing16A R; Supplementary Table 5).
Three optimal combinations of selective bases at the 3′ end of the designed primers (i.e., CC, GG, CG) could be determined.
The adaptor primers i.e., the 5′ forward primers in RT, 1st-PCR and 2nd-PCR were depicted earlier (Ozawa et al., 2006).
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