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On the lagging strand, the polymerase repositions to a newly primed site every ∼1000 bp.
The polymerase is released from clamp and DNA and can be repositioned to a newly primed site to reinitiate DNA synthesis.
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The symbol _ and the underlined bases represent the end and the beginning of the GP5+ PCR priming site, respectively.
The allele-specific reverse primer includes both the flanking sequence reverse primer and the sequencer-specific reverse priming site.
These discrepancies were likely attributable to one of the barcodes being lost because of sequencing error in either the barcode or the PCR priming site.
Standard MSP will, however, be superior to MCA-Meth in cases in which priming site methylation is homogeneously found and reliably predicts gene silencing.
Possibilities include secondary structure, mutated priming sites, long poly A tail, or other homopolymer stretches near the priming sites on the template.
Candidate SCPCHs are categorized according to their content in priming sites flanking phylogenetic informative regions.
These primers, BA1168 and BA1817, also contained the P1 and P2 priming sites for pCLF3, respectively.
Distance parameter between forward and reverse priming sites can be adjusted by the user (default: 400 nt).
Priming sites and ambiguous regions of the alignment were excluded.
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