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However, these random primed methods produce non-specific amplification.
Random primed methods rely on PCR protocols, which are faster and simpler than ligation-mediated methods.
However, non-specific products are also often amplified when using the random primed methods.
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The Math5 probe was gel-purified from clone JN4C after digestion with XhoI and EcoRI, and was labeled to high specific activity with 32P--dCTP using the random hexamer (dN6) priming method [76].
The first strand cDNAs were synthesized from 5 μg of day 25 porcine placental total RNA using a 1.5 μM random pentadecamer priming method [ 47] and SuperScript® III First-Strand Synthesis SuperMix reagents (Invitrogen, Carlsbad, CA USA) in a final volume of 20 μL.
Plus, when a company is hacked via e-mail, it loses one of its prime methods for contacting its customers.
So we first devised a model suitable for both priming techniques (Methods; formula (2)), which reports the presence of the physical connection between expressed TARs and known exons.
The canal was effectively closed down by 1843, just as railroads were beginning to take over as the prime method of transportation.
"Yes," Mr. Yung said, admitting that describing what a posset is, rather than how it tastes, was not the prime method of salesmanship.
Use of cross-coupling latch resistors is a prime method of mitigating single event upsets (SEU).
Compared to the rank method, the prime method achieves a higher provenance compression ratio.
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