Sentence examples for prime synthesis from inspiring English sources

A break would be processed by RecBCD to create 3' ends which prime synthesis from a homologous DNA molecule.

The secondary structure of the ITR is necessary to prime synthesis of the second strand to allow transcription of the viral genes [2].

This will occur frequently if reverse transcription is primed from random hexamers, but is expected to be less common when oligo dT) is used to prime synthesis from the poly(A) tail.

Finally, one additional breakage cycle would occur at the amplified region allowing a BIR event to be initiated by a DSB in a YNLWTy1-2 elementoto prime synthesis at the intact chromosome XIV YNLWTy1-2 elementothetelomeremere accounting for the duplication of this last region (Figure 3E).

Transcription from LSP generates an RNA molecule (7S RNA), which is thought to prime synthesis of 7S DNA (39, 40).

The probes were labeled with [α-32P]-deoxycytidine triphosphate by random prime synthesis using Amersham Rediprime II Random Primer Labelling System (GE Biosciences).

The genes for expression of C/EBP LZ and Arnt basic region (with portion of Arnt Helix 1) were constructed from two unique oligonucleotides with 21 bp overlap by mutually primed synthesis [45] and amplified with terminal primers by use of the Advantage 2 PCR Kit, following the manufacturer's instructions (Clontech).

Reviewer #3: Using cell-free influenza polymerase derived by micrococcal nuclease treated vRNPS purified from virions the authors are unable to detect unprimed vRNA synthesis using a 53-mer cRNA template, whereas ApG primed synthesis of vRNA is observed.

The problem that the mammalian replication machinery encounters at the telomere is that the primers used to initiate DNA synthesis are comprised of ~12 nucleotides of RNA which, at least in theory, are removed shortly after they primed synthesis of the last telomeric Okazaki fragment.

For probe synthesis, the L. braziliensis α-tubulin gene was released from clone pTOLb α tub-B by BamHI/ PstI cutting, excised from gel, purified using Wizard® SV Gel and PCR Clean-Up System (Promega, Inc., Madison, WI, USA), and finally labeled with digoxigenin by randomly primed synthesis using the DIG High Prime DNA Labeling kit (Roche, Inc., Mannheim, Germany).

Various enzymes are added to allow the oligonucleotide to prime the synthesis of a complete strand within the vector.