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(Some 1.4 million of DirecTV's customers are subscribers of Prime star, which DirecTV acquired last year).
A master blogger and Twitterer, she has established herself as a prime star for the social media age.
Nastya Fomina, who was smoking with four teenage friends at Prime Star, a deli-like cafe near the Kremlin, said it disturbed her when passers-by asked for money.
Prime STAR GXL DNA polymerase (TaKaRa) was used for reaction.
For the exponential reaction, a total PCR reaction volume of 20 μL was prepared containing 10 μL of prime STAR Max DNA polymerase (TAKARA Japan), 1 μL of primer F389 (20 pmol), 0.5 μL of primer LAD1 (10 pmol), 0.5 μL of LAD3 (10 pmol), 1 μL of template and 7 μL of ddH2O.
For the linear reaction, a total PCR reaction volume of 20 μL was prepared containing 10 μL of prime STAR Max DNA polymerase (TAKARA Japan), 5 μL of primer F389 (100 pmol), 1 μL of genomic DNA (about 100 ng) and 4 μL of ddH2O.
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If you're heading to a prime star-spotting locale, look for taster experiences.
Outside Oscar-time, it's still prime star-spotting territory since most of the major studios use the Four Seasons as their preferred place to allow stars to give interviews to journalists.
That, combined with Mr. McIndoe's reputation as a good host from his days as a maître d' at Joe Allen, the venerable theater hangout, has helped make his establishment a prime star-gazing spot.
But Ms. Reynolds also said she owed her career to Kelly, who in his prime starred, directed, choreographed and even helped write some of his movies.
Bette Davis, in her prime, stars as Fanny Trellis, a vain, beautiful woman who marries a banker (Claude Rains, above, with Davis) whom she doesn't love.
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