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For example, a pound of beef steak with a USDA Prime label will cost more than a pound of beef steak labeled USDA Choice or USDA Select.
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A randomly 32P-labeled (Rediprime II Random Prime Labelling System, Amersham Biosciences) restriction fragment encompassing all of exons 1 and 2, all of the ORF and a part of exon 3 (XbaI-fragment) was used as a PrP probe.
A specific probe was obtained by BglII digestion of transgene caDGK cDNA and [ α-P]dCTP-labeled (Amersham Rediprime Random Prime Labeling, Little Chalfont, UK); the membrane was hybridized with ExpressHyb (Clontech, Mountain View, CA, USA).
Probes were P-dCTP-labelled using Rediprime II Random Prime Labelling System (Amersham) according to manufacturer's instructions and purified with spin columns (Amersham).
DNA probes were labeled with 32P-labeled using the Rediprime-II Random Prime Labeling System (Amersham Biosciences, Piscataway, NJ) according to the manufacturer's protocol.
The membrane was hybridised with 100 ng of fluorescein-labeled probe prepared using the Random prime labeling module (Amersham Biosciences).
Hybridization with the P-labeled DNA probe (Rediprime II Random prime labeling kit, Amersham #RPN1633) was performed in Perfect Plus Hybridization buffer (Sigma #H7033) at 65°C overnight.
The membrane was hybridized with 100 ng of fluorescein-labeled probe prepared using the Random Prime Labeling Module kit from Amersham.
Probes of interest were prepared by PCR (Table 3) and then radio-labeled using αP32dCTP with Amersham RediPrime Random Prime Labeling System (GE Healthcare).
The membrane was hybridized with 100 ng of fluorescein-labeled probe prepared using the Amersham "Random prime labeling module" kit.
Fifty nanograms of PCR product amplified using the primer sets employed in RT-qPCR served as template for the synthesis of fluorescein-11-dUTP-labeled probes using the Gene Images Random Prime Labeling kit (GE Life Sciences, Uppsala, Sweden) according to the manufacturer's instructions.
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