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(I ) Survival of BALB/c mice that were primed, boosted and challenged as the C57BL/6 mice described above.
Ad-H4, rH4, the hetrologous prime boost combination, non-vaccinated, and BCG vaccinated mice were included.
The trial vaccination regimen was a candidate prime boost malaria vaccination.
In contrast, the heterologous prime boost combination resulted in augmentation of both the CD4 and CD8 response.
The protective effect of α-crystallin based prime boost vaccination was further substantiated by pathological observations in Exp-II.
The pathology and cytokine expression data from guinea pigs support the protective potential of α-crystallin based prime boost approach.
We next examined the influence of prime boost vaccination on pathological changes in various organs of guinea pigs.
We used two different prime boost regimens for aged mice.
This supports the prime boost strategies being employed for some new TB vaccines currently under development.
By day 5 after infection, the prime boost group of young mice regained weight 2-3 days prior to control mice.
The authors would like to thanks all members of the European Prime Boost Consortium, contract number QLK3-CT-2002-02010.
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