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For measurement of tumor tissue OPN, it was possible to obtain primary tumor blocks/slides for OPN IHC in 462 (69%) of 667 patients accrued to MA.14.
This is a major difference between CTCs and biomarkers from paraffin-embedded primary tumor blocks, for which real-time processing is not mandatory.
Seventy-two primary tumor blocks were initially available from patients who had received no prior anticancer treatment and 14 from patients with recurrent tumors (four paired samples).
Formalin-fixed, paraffin-embedded (FFPE) primary tumor blocks from participating patients were retrospectively collected from the archives of pathology departments throughout hospitals of Denmark.
Tissue samples may be in the form of paraffin-embedded primary tumor blocks from which cores may be taken to create tissue microarrays or up to 15 unstained microscope slides cut from those blocks.
The current study was intended to be complementary, and extend our investigations to early breast cancer taking advantage of the infrastructure of an existing prospective clinical trial that included collection of primary tumor blocks, and serial blood samples.
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For most patients (n=549; 86.9%), KRAS analysis was conducted on the primary tumor block.
In preclinical animal models, systemic administration of MRX34 inhibited the growth of primary tumors, blocked metastasis, and extended survival.
DNA was extracted, from ≥2 primary tumor FFPE blocks per patient.
Archival FFPE primary tumor tissue blocks from patients enrolled in the trial were retrospectively collected between January 2006 and December 2010 from study sites and centrally stored.
Briefly, paraffin-embedded primary tumor specimen blocks were sectioned at 10 μm and deparaffinized in 100% xylene, followed by a 100% ethanol incubation, and stained with hematoxylin and eosin.
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