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The jump happens when the thermophoretic force becomes either strong enough to overcome the potential barrier of the primary optical trap and push the particle upward to the secondary trap (forward case) or weak enough to allow the particle to settle into a position where it is mainly under the influence of the primary trap (reverse case).
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Even slightly higher amounts of imbalance would be buffered by the terD and terB sites flanking the primary fork trap.
We constructed and tested a bipartite GBT vector with Gal4-VP16 as the primary gene trap reporter.
The use of Gal4-VP16 as the primary gene trap reporter may also raise some concerns.
We do not favor this strategy, since there appears to be a positive side effect of using Gal4-VP16 as the primary gene trap reporter.
Another potential concern for using Gal4-VP16 as the primary gene trap reporter is that expression of UAS-controlled transgenes are susceptible to variegation and silencing in zebrafish [ 47, 66].
This conclusion was shown to be in agreement with predictions based on chemical kinetics, which were then used to support the proposal that the primary species trapped in the lipid vesicles were radicals derived from membrane fatty acids.
The crystal structure of cryoreduced oxy-CPO indicated that the primary species trapped at 90 K is a hydroperoxy rather than peroxy ferriheme intermediate.
As noted above, the primary products trapped during cryoreduction of crystal and frozen solution are frequently not the same, presumably because of differences in structural details of the oxyheme site in the crystalline form and in frozen solution.
In the primary forest, traps were set up at five different heights above forest ground (1 m, 8 m, 16 m, 24 m, 32 m) and in the secondary forest at three different heights (1 m, 8 m, 16 m; Fig. 1).
It is predicted that no primary dislocations are trapped at the habit plane.
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CEO of Professional Science Editing for Scientists @ prosciediting.com