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Primary templates were taken from A. mellifera.
The T. cruzi maxicircle coding regions provide the primary templates for the RNA editing process.
Amplification based on candidate EST primary templates was efficient, with only 13% of LAP pairs failing to generate amplicons.
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Structural sizes in 3D ZnO structures obtained through templating processes depend highly on the sizes of the primary template.
To verify that plus strands were indeed the primary template for the PCR reactions, we incubated core DNA with sodium hydroxide to remove the RNA primer attached to the 5' end of plus strand DNA, thus removing 12 nts of the annealing site for primer 2477 during the PCR reaction (Table 1, experiment 5, Figure 3).
Codegen utilizes Java-related skill-sets by using Java Emitter Templates (JET) as its primary template language[ 17].
In mammalian cells, the sister chromatid is the primary template for HR compared to the homologous chromosome [ 20].
A total of 14 primer pairs amplified paralogous sequences, at a mean of 2.5 per primary template sequence, with a range from 1 12.
For second strand synthesis, PCR was carried out on a small aliquot (1/10th volume) of the primary template by using Advantage 2 Polymerase Mix (Clontech).
However, these results were based on qualitative RT PCR, and differences in primer sensitivity and primary template concentrations could affect the levels of amplified product.
For second-strand synthesis, PCR was carried out using a small aliquot (1/10th volume) of the primary template and Advantage 2 Polymerase Mix (Clontech).
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