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Because of a major technical limitation in studying primary cultured stellate cells that consistently have very low transfection efficiency typically less than 1% [23] we used LX-2 cells that display a relatively high transfection efficiency of >30% using a commercial reagent [23].
"That's a technical limitation".
Results: primary technical success was achieved in 39 cases (78%).
(The biggest technical limitation for mobile devices now is battery technology, which has not seen a true breakthrough in decades).
Note that Twitterspy's delay is not a technical limitation but rather a business relationship limitation.
Primary technical success rate was 100%.
Q: What is the biggest technical limitation outside your control in this field?
There's also a technical limitation which isn't often discussed in the media.
Was this a technical limitation or something that grew out of a design decision?
Several approaches have been pursued to overcome this technical limitation.
Several techniques have been developed to circumvent this technical limitation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com