Plant height and the number of siliques on the primary stem were measured at the cessation of flowering; the longest stem was used for plants with multiple primary stems.
For oilseed hand pollinated plants, the first 30 flowers to develop on the primary stem were supplementary pollinated, with pollen from 5 donor plants.
Five internodes, i.e. the aforementioned internodes 2, 3, 5, 7, and 9 from the Medicago primary stem, were collected in three biological replicates.
The length of the primary inflorescence stem was significantly increased in the 35S ARR1 D94E (L) line, which is a likely consequence of the delayed senescence phenotype observed in these plants.
For light microscopy, the lower ~5 mm of the primary inflorescence stem was fixed in formaldehyde/glutaraldehyde buffer (3.5% and 0.5% v/v, respectively) for up to five days and dehydrated in an ethanol series before embedding in LR White™ resin.
Total RNA extracted from the bottom 100 mm of the primary inflorescence stems was treated with the RNase-free DNase Set (Qiagen) and genomic DNA contamination assessed by PCR using intron-spanning primers.
The indication for using the short stem was a primary tumor in seven patients, aseptic loosening of a distal femur replacement in five, and local recurrence or intralesional resection in four (Table 1).
A time-dependent transition rate might for example be encountered in an in vitro stem cell system, where primary stem cells are isolated, separated from the stem cell niche.
In most cases, primary THR stems were used with metal-on-polyethylene or ceramic-on-ceramic bearings.
Flowering time was recorded as number of rosette leaves and days from germination when primary inflorescence stems were 5 mm long.
