Sentence examples for primary stained from inspiring English sources

Controls for immunofluorescent stainings were taken at the same or greater exposure time as primary stained samples.

The corresponding lung primary stained positive for CK7, and negative for CK20.

After washing in PBS, primary stained monolayers were reacted with corresponding fluorescent-conjugated secondary antibodies (Sigma-Aldrich, St . Louis MO) diluted in 3% BSA-PBS.

Ninety-six per cent of the primary lung lesions were CK7+/CK20−, while 100% of colorectal primaries stained CK7−/CK20+.

Biopsies from all 10 patients with lung primaries stained CK7+/CK20−, while all metastatic lesions from the colon were CK7−/CK20+ (Tan et al, 1998).

Primary staining was followed by incubation with secondary antibodies conjugated to fluorochromes.

Secondary antibody staining for FITC conjugates is significant but different than most of the primary staining of interest (Figure 2; panel O).

To test label-free unbinding the primary staining was done with unlabeled phalloidin and ph-A488 at a ratio of 4 (unlabeled):1 (labeled).

After primary staining, the cells were washed three times then secondary antibodies (streptavidin-PE, streptavidin-APC, or streptavidin-APC-Cy7) were added and cells were incubated for 30 minutes at 4°C.

Primary staining was done with periodic acid-Schiff (PAS).

A no primary staining control (staining without NF-κB antibody) demonstrated antibody immunospecificity.