Sentence examples for primary spheres of from inspiring English sources

To assess that the morphological changes are due to the microenvironmental factors, the primary spheres of SUM149PT and SUM1315MO2 were trypsinized and reseeded for the growth of secondary spheres and were observed after 10 days (Fig.  1d).

The active site of metalloproteases is composed of a primary sphere of residues co-ordinating the zinc atom, and a secondary sphere of residues that determines proteolytic specificity and activity.

Their primary sphere of influence comes from their role as wives and mothers.

For secondary colonospheres, the 96-well plate of primary spheres was centrifuged at speed of 1000 rpm for 1 min and the supernatant was removed.

As for primary spheres, the number of secondary spheres was determined under microscope.

Subsequent cultures after dissociation of primary spheres were plated in 1% coated plates at a density of 1000 cells/ml.

The number of primary spheres was counted after 7 12 days in vitro (DIV).

For serial passage of primary spheres, the primary spheres were treated with PSP for the above doses for 72 h and subsequently collected, dissociated with trypsin, and resuspended in DMEM/F12 medium with the above supplements.

The number of primary spheres was counted under microscope.

After 7 days from primary culture the numbers of primary spheres were counted under a microscope (Additional file 1: Figure S2) [ 21].

Primary spheres obtained after 11 days of culture in the presence of HGF were dissociated and 5000 cells were replated and grown either in the presence or absence of HGF for an additional period of 10 days.