The process continues until we get the ten intelligences and nine primary spheres that make up the standard picture of medieval cosmology.
Small Angle X-ray Scattering and Static Light Scattering were used in order to provide information on the size of the primary spheres and the aggregates respectively.
The mass specific extinction coefficient and the polarization ratio at 90° of simulated smoke agglomerates are computed for clusters with 3 to 1390 primary spheres and for size parameter from 0.05 to 0.6 using the coupled electric and magnetic dipole (CEMD) method.
For the size parameter range of interest for postflame smoke, the specific extinction coefficient computed by CEMD is 21%to36%6% greater than Rayleigh prediction, and for this size range for clusters with 100 to 1000 primary spheres, the range in the specific extinction coefficient is about 12%.
These primary spheres were harvested and replated in fresh media.
The number of primary spheres was counted after 7 12 days in vitro (DIV).
For serial passage of primary spheres, the primary spheres were treated with PSP for the above doses for 72 h and subsequently collected, dissociated with trypsin, and resuspended in DMEM/F12 medium with the above supplements.
Primary spheres obtained from CD24− cells could be enzymatically dissociated with trypsin into single cells to give rise to secondary spheres (data not shown).
Thus, the percentage of MeCP2 expressing cells was initially higher in primary spheres but adjusted to the expected 50% level after 5 passages.
Primary spheres were dissociated and passaged in the secondary culture and similar results were obtained (mean SFU in secondary culture is 1.39±0.20% for the control group and 2.72±0.50% for the Y-27632 group, n = 3, P = 0.012).
Transduction of these cells with Retro-EF1α-EGFP resulted in EGFP expressing neurospheres that continued to express EGFP for 10 passages (Figure 5, green line) with comparable expression to primary spheres infected at the 10th passage (39.4% and 34.8% respectively, Figure 5, upper right).
