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The primary solution was diluted with 0.9% saline to obtain the various treatment solutions and administered under the same volume by daily gavage.
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How seriously can you take anyone complaining about debts and deficits at this moment when his primary solution is to lower tax rates beyond the Bush levels and starve the government of revenue?
Statistically, this problem can be regarded as "weak power," for which the primary solution is to increase the number of individuals in the study.
Higher-order corrections to primary solutions are proposed through an iterative procedure.
The primary standard solution was prepared by appropriate dilution of 1,000 ppm standard solution in accordance with the selected wavelength and sensitivity check.
The primary antibody solution was then exchanged with 5% goat serum, and surfaces were counterstained with a secondary Alexa-Fluor 647 IgG antibody (ab150131, abcam) in 6% goat serum for 1 h.
Primary antibody solution was then aspirated and cells washed 3 times in PBST for 5 minutes each wash.
Control sections in which the primary antibody solution was replaced by blocking solution did not show any signal in the seminiferous tubules (Fig. 2D).
Primary antibody solution was washed off with 3 changes of PBST for 5 minutes each and then incubated with secondary antibody (AlexaFluor 488 or 555, Invitrogen) 1∶200 in blocking solution.
The primary antibody solution was diluted (1 5) in blocking solution containing 5% normal goat serum (NGS).
The primary antibody solution was removed and the cells washed several times with blocking buffer.
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