Aiming at current problem of being no basis for LNG terminal fire prevention system design, this article puts forward the primary solution on fire prevention design for such sites by combining fire risk of LNG terminal, requirements of relevant regulations both at home and abroad and practical experience.
Statistically, this problem can be regarded as "weak power," for which the primary solution is to increase the number of individuals in the study.
After incubation with primary antibodies, the slides were washed in TBS and incubated in post primary block solution for 30 min, followed by washes in TBS and incubation with the polymer solution for 5 min.
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Next day, the membranes were incubated with primary antibodies solution for 2 h at room temperature (RT) and washed using PBST (5-10 min) for three times, then incubated for 1.5 h at RT with secondary antibodies solution: goat anti-rabbit IgG H + L) HRP(1 12 000; Kangchen, CA), rabbit anti-goat IgG H + L) HRP(1 10 000; Kangchen, CA), goat anti-mouse IgG H + L) HRP(1 10 000; Kangchen, CA).
After craniotomy, the brains were post-fixed in the primary fixative solution for 48 h and cut into 150-µm thick sections with a Vibratome (Micron International GmbH, Walldorf, Germany).
For cryosections, slides were incubated for 30 minutes in blocking buffer (same as above) at room temperature, and then incubated with primary antibody solution for 16 hours at 4°C, followed by incubation with secondary antibody and DAPI for 2 hours at room temperature.
Embryos were incubated in primary antibody solution for 45 minutes at room temperature.
