Sentence examples for primary sheep from inspiring English sources

As a result of cross-reaction between secondary antibodies with primary sheep and goat antibodies, co-staining HAI-1 (goat) with matriptase (sheep) was not possible.

The primary sheep polyclonal antibody to LDH5 isoenzyme (ab9002, Abcam, Cambridge, UK) was applied at a dilution 1 : 200 overnight at 4 °C.

Bound primary sheep AB to ETAR and ETBR were detected using DAKO Rabbit-EnVision-HRP, via bridge AB rabbit-anti-sheep (Dianova, Hamburg, Germany, 1 : 50).

ASMC were fixed with 4% formaldehyde for 10 minutes, permeabilized with methanol (−80°C), blocked with 2% bovine serum albumin (in phosphate buffered saline), and incubated with a primary sheep monoclonal antibody specific to α-actin and rabbit monoclonal antibody specific to TRPV1(1 100, Santa Cruz Biotechnology Inc., USA) overnight at 4°C.

The intracellular targeting primary rabbit anti human Kirrel 3 antibody (102960 Abcam Cambridge UK) and the extracellular targeting primary sheep anti Kirrel 3 antibody (AF4910 R and D systems Minneapolis USA) were incubated over night at room temp with gentle agitation in 1× TBST with 5% semi skimmed milk.

Following blocking with TBS (pH 7.5) containing 0.1% (v/v) Tween 20 and 5% (w/v) non-fat dried milk for 2 h at room temperature, membranes were hybridised at 4 °C overnight with the primary sheep polyclonal anti-LDH5 antibody (1 : 5000, Abcam; cat. no.

After this, sections were incubated for 48 h in a mixture of the following primary antibodies: sheep anti-cGMP (1 4000) and mouse anti-GAD65 (1 250) diluted in TBS.

Cells were then blocked with 5% Donkey Normal Serum (Sigma-Aldrich) in 0.3% PBS/Triton-X 100 (Merck, Darmstadt, Germany) for 1 h at room temperature, then incubated overnight at 4°C in humid atmosphere with the primary antibody (sheep anti-Cyt-C: 1 800; Sigma-Aldrich) diluted in blocking solution.

His main challenger in the primary, Bill Taliaferro, a sheep rancher, hammered him relentlessly on the matter.

Next, the sections were incubated overnight at 4°C with the primary antibody (polyclonal sheep anti-SOD1 antibody at 1∶500) in 0.1 M PBS containing 0.3% Triton X-100 and 3% BSA.

Primary cultures of sheep pleural mesothelial cells were prepared (modified from Stylianou et al [ 32]).