Exact(1)
Indeed, other studies that based their primary screens on a positive PA-binding signal in direct ELISA format were able to select only 3 10 antibodies per screen [9] [14].
Similar(58)
They consciously want to connect with movie goers via a primary screen, on which you presumably watch the movie, and a secondary screen, on which you do something other than watching said movie.
We identified inhibitors of Candida growth formation by performing a primary screen on the 1581 drugs belonging to the JHCCL.
We focused our primary screen on Synaptojanin 1 and OCRL, and identified two scaffolds with distinct specificity profiles.
When superior isolates passing the primary screen on PSGHL were evaluated on the three hydrolyzate types (SGH-N1, SGH-N2, and AFEX CSH), the overall mean xylose uptake rate across isolates varied significantly between hydrolyzate types (P < 0.001): 0.24 g/L/h for SGH-N1, 0.19 g/L/h for SGH-N2, and 0.12 g/L/h for AFEX CSH.
Then, primary screening of the isolates was performed on 2%% (w/v) malt extract agar plate containing 0.01 % (w/v) ortho-dianisidine and guaiacol as a chromogenic substrate to check the presence of polyphenol oxidase activity (Ravikumar et al. 2012).
HeLa H2B GFP cells were transfected with the four individual duplexes, and also the pool of 4, at 15 nM total siRNA, a lower concentration than the primary screen to further select for on-target effects.
miRNA inhibitors that demonstrated Z-score values that were two standard deviations (SD) from the control were selected as hits and were included in follow-up experiments (Fig. 1a).Of 396 miRNA inhibitors, fifteen were identified in the primary screen for their effect on differentiation.
Whether our results can be extended from a primary screening to a triage population depends on the performance of the cytology tests on (a) fluid remnant after primary HPV testing in HPV-positive women (in case of reflex triage testing) and (b) directly taken material in (previously) HPV-positive women (in case of triage testing at 6 months).
The use of the V79-4 system as a primary screen for fiber carcinogenicity is dependent on the observed correlation between cytotoxicity of the test material in the system and mesothelioma following intrapleural injection in animals.
9 Several randomised controlled trials are currently being conducted to compare primary screening based on HPV detection with conventional cytology screening.
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